Design of Microscopy-based Microcontact Printing for Structural Patterning of Neuronal Cells

• Main Authors: Tsung-Liang Chuang, 莊琮亮
• Other Authors: Jia-Jin Chan
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/57086414022121593468

碩士 === 國立成功大學 === 醫學工程研究所碩博士班 === 92 === 　　To culture neuronal cells grown in a geometric pattern on a modified substrate plate is essential to understand the neuronal cell behavior. Recent studies have made it possible to control neuronal cell positioning as well as outgrowth by using the microcontact printing technique. The aim of this study was to design an inexpensive microscopy-based microcontact printing device for aligning the pattern to the substrate for guiding neuronal growth. In the study, the microfabrication of the microcontact printing stamp of Polydimethylsiloxane (PDMS) was used to define the cytophilic region by transferring poly-D-lysine (PDL), cell adhesion factor, to surface-modified substrate. The cytophobic region was then developed by immersing the printed PDL substrate in bovine serum albumin (BSA). Our study examined the concentrations of PDL to form cytophilic region and provides a micro contacting scheme to produce a reliable structural pattern on the substrate. Furthermore, we investigate the relationships between the ink concentration and printing force monitored from torque sensor to the peptide pattern from fluorescent image of printing lines. Further studies are needed to systematically investigate the suitable ranges for varied ink concentrations. It is believed that our device with some further modification can provide an inexpensive microcontact device to study neuronal behaviors, i.e. neuronal patterning and network/synapse formation. Too low in applying force might cause the poor transfer rate but the too high in applying force might cause the deformation of stamp due to the low ratio of depth to width of the stamp. However, this result is affected by the PDL concentration applied.