| Summary: | 碩士 === 國立成功大學 === 藥理學研究所 === 92 === Antrodia camphorata, or ”chang-chih” in its folk name, is a tree fungus that has been used by Taiwanese indigenes for years for its anti-inflammatory activity. Several compounds have been identified from the fruiting body of Antrodia camphorate, such as triterpenoids, sesquiterpenoids, steroids, and polysaccharides. Previous researches indicated that zhankuic acid extracted from fruit body of A. camphorata exhibited cytotoxicity against P388 murine leukemia and aqueous extract of A. camphorata reduced the viability of HL-60 human leukemia cells. And yet no animal experiments and other aspects of anticancer activities of A. camphorata have been tested. My study aims at investigating the anticancer activity of a methanolic extract obtained from cultured mycelia of A. camphorate, termed ACME.
The extract shows markedly dose-dependent cytotoxicities against several cancer cell lines. The flow cytometry and DNA fragmentation assays demonstrate that the cytotoxicity is due to cell apoptosis. The anticancer activity of ACME was investigated further in vivo using mouse bearing transplanted tumor cells. ACME significantly inhibits the growths of tumors both in the Lewis lung carcinoma and ML1-4A hepatoma animal models. Besides, we found ACME inhibits the migration of human prostate cancer cells (PC-3) toward chemo-attractant and reduces MMP-9 but not MMP-2 secretion, suggesting ACME possesses an anti-metastatic principle. ACME also exhibits anti-angiogenesis activities in an endothelia cell migration assay and blood vessel formation in a semi in vivo chicken chorioallantonic membrane (CAM) assay. But no apparent differences are found in CD31 immunohistochemistry of ML1-4A primary tumors from either control animal or animal treated with ACME, indicating the tumor-inhibiting activity is not due to angiogenesis inhibition.
The active anticancer ingredient and its detailed molecular action mechanism in A. camphorate warrant further investigation.
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