The study of Linker for DNA Immobilization on the Surface of a Gene Chip

• Main Author: 林建輝
• Other Authors: 祁甡
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/84190563483512740838

碩士 === 國立交通大學 === 光電工程系所 === 92 === With the coming of the age of rapid DNA sequencing, gene-chip technology has already been an essential tool for the life science researches. Because of the compact and high density features, we are capable of mining massive genetic information. Moreover, the sensitivity of gene chip manufactured by semiconductor technology achieves to distinguish single nucleotide variation. Nowadays, gene chips are not only applied in the field of life science and drug development but will change the current clinical diagnostics and bring the revolution of civilization in the near future. In the decade, the general technique of fabricating gene chips adopts glass for the substrate of gene chips and silane for the linker. However, the poor properties of glass and silane limit the reliability of gene chips. In this thesis, we develop a method of utilizing an alkene material for DNA in situ synthesis on a silicon wafer, and the feasibility of this method has also been demonstrated. We believe that this method of immobilizing DNA on silicon wafer is capable of advanced applications on micro-electromechanical sensing devices.