| Summary: | 碩士 === 國立中央大學 === 生命科學研究所 === 92 === Abstract
Galectin-1 (GAL1, a b-galactoside-binding protein) functions in cell adhesion, development, and growth regulation, but the mechanism of GAL1-induced cell differentiation remained to be defined. In this study, we showed that coating of GAL1 in culture plates promoted the differentiation of 3T3-L1 preadipocyte, which can be dose-dependently inhibited by GAL1 inhibitor, lactose (50-200 �慊/ml) and TDG (10-30 mM). Fluorescence-activated cell sorting (FACS) analysis showed that GAL1 treatment did not induce mitotic clonal expansion of adipocytes, in which has been thought to be an essential preliminary step of adipocyte differentiation. In addition, expression of dominant negative C/EBP α and C/EBP β showed no effect on GAL1-mediated 3T3-L1 differentiation. These results suggested that GAL1-mediated 3T3-L1 preadipocyte differentiation is likely through C/EBP��- and C/EBP��-independent pathway. Thus, it is evident that the mechanism of GAL1 action on 3T3-L1 preadipocyte differentiation seems distinct from that induced by IDM.
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