Studies of Ginsenosides on Glucose Absorption in Caco2 Cells and Intracelluar ATP Level in HepG2 Cells

• Main Author: 楊德群
• Other Authors: 張溫良
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/02913673854393858953

碩士 === 國防醫學院 === 藥學研究所 === 92 === Ginseng, the root of Panax ginseng, is a very old and well-known drug in traditional Chinese medicine used mainly to reinforce qi and invigorate the function of the spleen. Being interested in exploring of the mechanism of reinforcement qi of ginseng, the investigation of P. notoginseng, the same genus of ginseng, was undertaken in this study to prepare ginsenosides which were mainly active components of ginseng. The alcohol extract of the root of P. notoginseng was separated and purified by adsorbent resin, silica gel and reversed-phase chromatography to give five known ginsenosides, ginsenoside Rb1 (1), Rg1 (2), Rh1 (3), Re (4), and notoginsenoside R1 (5). Then, ginsenoside Rb1 and Rg1 were hydrolysis by naringinase to yield metabolites, ginsenoside F2 (6), compound K (7), as well as ginsenoside F1 (8) and 20(S)-protopanaxatriol (9). These compounds were identified by comparison of their physical and spectral data with those reported in the literature and authentic samples. The glucose absorption and ATP assay were used as bioactivities experimental models. The glucose absorption could be increased by NL-WLC-P01, P02, P03, P04, P06, and P08 in Caco 2 monolayer system, while NL-WLC-P02 especially enhanced 104% glucose absorption at dose of 0.001 μM, whereas, NL-WLC-P05 (0.001 μM) and P07 (0.01 μM) decreased 50% glucose absorption. NL-WLC-P02 could enhance about 30% ATP contained in HepG2 cells. The mechanism of these effects remains to be clarified.