Biodegradation Enhancement by Screened Pentachlorophenol Degradating Bacteria
碩士 === 國立屏東科技大學 === 環境工程與科學系 === 92 === The widespread use of the pentachlorophenol (PCP) has caused certain pollutions in the soil, water, and air. However, Bioremediation is an economical and feasible disposing way that helps to reduce the damage to the environment. This study isolated the PCP deg...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2004
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| Online Access: | http://ndltd.ncl.edu.tw/handle/18937456832357902760 |
| Summary: | 碩士 === 國立屏東科技大學 === 環境工程與科學系 === 92 === The widespread use of the pentachlorophenol (PCP) has caused certain pollutions in the soil, water, and air. However, Bioremediation is an economical and feasible disposing way that helps to reduce the damage to the environment. This study isolated the PCP degradating bacteria from three different PCP degradation microbial consortia in compost-soil that we have been climated for 150 days, and to find the most suitable environmental parameter on degradation. Then we used bioaugmentation to add screened bcteria into microbial consortia for enhancing PCP degradation activity.
The diversity of the three microbial consortia analysis by denaturant gradient gel electrophoresis (DGGE) showed the same structure in mature hog manure and bark compost-soils and they were more diversity than the structure of contaminated-soil only. Enriched more times with PCP added, the diversity of PCP degradation microbial consortia became simpler. Pseudomonas citronellolis NPUED001 was isolated from bark compost soil and it showed 24.3 % PCP degradation ratio in the 75 mg/L concentration of PCP. The most suitable degradation pH for PCP degradation by NPUEM001 is 7.88~8.99 under the temperature of 30~35℃ with 75 mg/L initial concentration of PCP. The higher inoculum density of NPUEM001 will
increase the PCP degradation ratio. The most efficient degradation ability is 51.41 % when the inoculum density is 2.8×106 cell/mL in 75 mg/L PCP-concentration. The initial concentration needs to reach the rang within 1.4×106~2.8×106 cell/mL of inoculum density for the effectiveness. When added with glucose as the second carbon source the PCP degradation ratio was raised up 17.3 % with 75 mg/L initial concentration of PCP but there was no obvious increase for citric acid addition. The bioaugmentation by added proliferation NPUEM001 into PCP degradation microbial consortia can effectively increase PCP degradation ratio to 50.7 %. The toxicity analysis of culture medium by Microtox showed the toxicity of PCP didn''t descend after the degradation of NPUEM001. The Pseudomonas citronellolis NPUEM001 and microbial consortia from bark compost-soil could be the inoculum for PCP contaminated site in Taiwan in the future.
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