The Expression of Phalaenopsis amabilisvar. Shimadzu Genes Selected from Subtration with High Temperature

• Main Authors: Jing-Ling Huang, 黃靜鈴
• Other Authors: Tsai-Yun Lin
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/yw6gpt

碩士 === 國立清華大學 === 生物資訊與結構生物研究所 === 92 === Abstract Phalaenopsis amabilis var. formosa Shimadzu is one of the common tropic and sub-tropic plants that possess some thermotolarance under heat stress. Despite of this capacity, however, the Phalaenopsis would easily be dormant or cease growing, and could be severely infected by pathogen during long-term high temperature. To determine how Phalaenopsis responses to high temperature, we constructed a heat-induced partial cDNA library using PCR-select subtraction. Within the 120 ESTs selected for this study, there are genes encoding the heat shock transcription factor and the small heat shock protein that are involved in known protective mechanisms at higher temperaure. In addition, we obtained some ESTs conding for proteins associated with auxin signal transduction pathway, such as AUX1 permease and Chac-like protein (cation transporter). Interestingly, Northern blot analyses indicated that the transciption levels of those ESTs seem to be regulated by circadian rhythm. Meanwhile, we analyzed the promoter region of these putative auxin-related ESTs to acquirte more information. Our results suggest that the auxin signaling pathway probably interact with heat sock response in Phalaenopsis.