| Summary: | 碩士 === 國立臺灣海洋大學 === 機械與輪機工程學系 === 92 === This thesis is to apply the atomic force microscopy (AFM) in the study of rapid diagnosis of immune response and dynamic reactions of an antimicrobial peptide (AMP) and germs for aqua cultured fish. Glass is used as the substrate, and self-assembled monolayers (SAMs) are produced on glass by chemical treatments to immobilize antibodies or germs. We first detected the specific binding between antigens and antibodies for rapid diagnosis of groupers using atomic force microscopy. We also investigated three-dimensional morphological changes of Escherichia coli, Vibrio harvyi, and Vibrio alginolyticus before and after treatments of monodoncin, an antimicrobial peptide (AMP) from tiger shrimp. Experimental results exhibit successful applications of AFM in studies of aquatic biology. AFM not only can be utilized for rapid diagnosis of immune response, but also can be used to visualize dynamic reactions of antimicrobial peptides and bacteria. It helps understand the sensitivity of bacteria to AMP and the effectiveness of AMP. As a result, AFM can be a great investigating tool in pharmaceutics for aquatic biology.
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