RNF4 enhances AP-1 response element activity
碩士 === 國立臺灣大學 === 生化科學研究所 === 92 === The small nuclear RING finger protein 4 (RNF4) contain C3HC4-type RING motif in the c-terminal region. RNF4 modulates both steroid-receptor-dependent and basal transcription and interacts with a variety of nuclear proteins. RNF4 can also act as a transcriptional...
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ndltd-TW-092NTU001030082015-10-13T13:27:34Z http://ndltd.ncl.edu.tw/handle/58503698761570964493 RNF4 enhances AP-1 response element activity RNF4可以促進AP-1反應單元的活性 Ching yin Liu 劉靜穎 碩士 國立臺灣大學 生化科學研究所 92 The small nuclear RING finger protein 4 (RNF4) contain C3HC4-type RING motif in the c-terminal region. RNF4 modulates both steroid-receptor-dependent and basal transcription and interacts with a variety of nuclear proteins. RNF4 can also act as a transcriptional co-repressor or co-activator. In this study, we demonstrated that RNF4 enhance AP-1 (activating protein-1) transcriptional activity. Using luciferase assay, we showed that RNF4 regulates AP-1 through c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinases (ERK) pathway. In addition, GST-pull down assay indicated that RNF4 can interact with p-JNK and p-ERK. When RING finger motif is deleted, RNF4 loses its ability to enhance AP-1 activity. T127, S166 phosphorylation sites of RNF4 is critical to activate AP-1 transcription. If the two phosphorylation sites mutated to Alanine, RNF4 lose the enhancement of AP-1 activity. Based on these results, we propose that RNF4 enhance AP-1 transcription activity. Yu-May Lee 李玉梅 2004 學位論文 ; thesis 59 en_US |
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碩士 === 國立臺灣大學 === 生化科學研究所 === 92 === The small nuclear RING finger protein 4 (RNF4) contain C3HC4-type RING motif in the c-terminal region. RNF4 modulates both steroid-receptor-dependent and basal transcription and interacts with a variety of nuclear proteins. RNF4 can also act as a transcriptional co-repressor or co-activator. In this study, we demonstrated that RNF4 enhance AP-1 (activating protein-1) transcriptional activity. Using luciferase assay, we showed that RNF4 regulates AP-1 through c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinases (ERK) pathway. In addition, GST-pull down assay indicated that RNF4 can interact with p-JNK and p-ERK. When RING finger motif is deleted, RNF4 loses its ability to enhance AP-1 activity. T127, S166 phosphorylation sites of RNF4 is critical to activate AP-1 transcription. If the two phosphorylation sites mutated to Alanine, RNF4 lose the enhancement of AP-1 activity. Based on these results, we propose that RNF4 enhance AP-1 transcription activity.
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author2 |
Yu-May Lee |
author_facet |
Yu-May Lee Ching yin Liu 劉靜穎 |
author |
Ching yin Liu 劉靜穎 |
spellingShingle |
Ching yin Liu 劉靜穎 RNF4 enhances AP-1 response element activity |
author_sort |
Ching yin Liu |
title |
RNF4 enhances AP-1 response element activity |
title_short |
RNF4 enhances AP-1 response element activity |
title_full |
RNF4 enhances AP-1 response element activity |
title_fullStr |
RNF4 enhances AP-1 response element activity |
title_full_unstemmed |
RNF4 enhances AP-1 response element activity |
title_sort |
rnf4 enhances ap-1 response element activity |
publishDate |
2004 |
url |
http://ndltd.ncl.edu.tw/handle/58503698761570964493 |
work_keys_str_mv |
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1717736015900180480 |