Effect of the ethanolic extracts from lotus leaf on the early stages of atherosclerosis

• Main Authors: Chi-Ying Lee, 李季螢
• Other Authors: Wenchang Chiang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/65576064008724942282

碩士 === 國立臺灣大學 === 食品科技研究所 === 92 === Atherosclerosis is a chronic inflammation disease that cannot be defined as a single disease entity. In early study, it shows atherosclerosis has great relationship with lipid accumulated in surface of vessel but recently research has indicated oxidative stress and oxidation of LDL(low density lipoprotein)being major causes, too. In this research, we use the ethanolic extract from lotus leaf(LLE)and its four partitions(LLEP1、LLEP2、LLEP3、LLEP4)as raw materials to study their effects on the early stages of atherosclerosis by in vitro test and cell model. The results showed that partition of ethyl acetate from LLE(LLEP2)had the greatest scavenging ability of reactive oxygen species and inhibition of LDL oxidation. LLEP2 could also promote macrophage ability to endocyte oxLDL and to decrease the inflammation which was induced by oxLDL and macrophage-derived foam cells. Consequently, it seems LLEP2 can affect the early stages to retard the formation of atherosclerosis. In the next part assay, we used LLEP2 as materials to do gel filtration and got 11 subfractions(1 ~ 11). According the results we found subfraction of 8, 9, 10 had better ability than that of LLEP2. The results of HPLC showed the possible active compounds in the subfraction of 8, 9, 10 were quercetin、p-coumaric acid、syringic acid and other flavonoids. The ability of lotus leaf to retard the formation of atherosclerosis could be from flavonoids and some unknown compounds.