| Summary: | 碩士 === 國立臺灣大學 === 動物學研究所 === 92 === Apoptosis is a genetically controlled multicellular organisms process to maintain homeostasis and remove the potentially dangerous cells. The host can exterminate the infected cells base on activating the apoptosis system and thus suppress virus reproduction. On the other hand, viruses could replicate themselves or cause persistent infection by regulating the apoptotic pathway to delay the death of host cell. The previous investigations indicate that apoptosis occurs in white spot syndrome virus (WSSV) infected Penaeus monodon. In this study, WSSV-carriers and WSSV-free shrimp were intramuscularly injected with WSSV or PBS vehicle as controls. Moreover, three independent specimens of stomachs and lymphoid organs (LO) were then collected in different time points to examine apoptosis incurrence and levels of WSSV DNA replication through TUNEL assay and WSSV in situ hybridization (ISH) respectively. The findings reveal that the TUNEL positive cells were higher in the LO than in the stomach at 72 hours after WSSV infection. In contrast, the ISH positive cells were lower in the LO than in the stomach. The results facilitate further studies relationship between apoptosis and WSSV replication.
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