Enantioseparation of Catecholamines and Structurally Related Compounds by Capillary Zone Electrophoresis Using Sulfated-β-Cyclodextrin as Chiral Selectors.

• Main Authors: Hsu-Tun Cheng, 鄭旭敦
• Other Authors: 林敬二
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/89611257204424526558

碩士 === 國立臺灣大學 === 化學研究所 === 92 === In this study, we focus on the enantioseparation of three catecholamines and three structurally related compounds with addition of sulfated cyclodextrins and the optimization of separation. The influences of buffer concentration and temperature on the migration behavior and enantioseparation of these cationic solutes with addition of sulfated cyclodextrins were investigated. The results indicate that, buffer concentration and temperature play important roles in the enantioseparation of these cationic solutes. In order to understand enantoseparation mechanisms of these cationic solutes also used three kind of neutral cyclodextrins as chiral selectors. Moreover, by mixing two kind of cyclodextrins, we found the reversal of migration order of norephedrine、synephrine and octopamine. The interaction of chiral analytes with chiral selectors was studied through the variation of mobility of norephedrine、synephrine and octopamine.