Visualization and Quantification of Oleic Acid and Iontophoresis Induced Transdermal Drug Transport by Laser Scanning Microscopy

• Main Authors: Tsung-Kai Chiu, 邱宗凱
• Other Authors: Chen-Yuan D0ng
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/09163312691020198570

碩士 === 國立臺灣大學 === 物理研究所 === 92 === Abstract Transdermal transport offer many advantages such as long-term drug treatment and minimially invasive delivery. However, the main barrier of skin, stratum corneum, the outermost layer of skin, reduces the efficiency of transdermal drug delivery. In previous studies, there are several methods have to beed shown to enhance the efficient of transdermal drug delivery, such as sonophoresis, an application of mechanical wave (several kHZ) on skin; electroporation, an application of pulse high volts current (hundreds volts/ms) on skin; iontophoresis, an application of low-level electric current on skin, and chemically enhanced delivery methodology, such as the use of oleic acid have been shown to enhance the efficient of transdermal drug delivery. First, we investigated the effect of iontophoresis to the transport of oleic acid, a comparison between treatment of oleic acid with anodal or cathodal iontophoresis is done. Furthermore, we investigated the synergistic effects of oleic acid and iontophoresis on transdermal drug delivery. Comparison between the delivery efficiency of oleic acid treatment, iontophoresis and oleic acid treatment during iontophoresis delivery and prior to iontophoresis is done. Laser scanning microscopy was used to visualize the distribution of two fluorescent model drugs: calcein, a multiply charged (-4), hydrophilic molecule; and nile red, a neutral, hydrophobic compound.