The Mechanisms of Ethanol Toxicity in Alcohol Dependence

• Main Authors: Sheng-Hui Tang, 湯勝輝
• Other Authors: Fu-Chuo Peng
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/99pvqm

碩士 === 國立臺灣大學 === 毒理學研究所 === 92 === The aim of this study is to examine the relationship between alcohol dependence and oxidative stress. The biochemical parameters and antioxidants status are measured among 76 patients with alcohol dependence. Nineteen healthy persons without drinking problem are recruited as the control subjects. The AST, ALT, γ-GT, T-Bilirubin, ALP, Cholesterol and uric acid showed significant increasing in the blood specimen of patients as compared with those of the control subjects (p < 0.05). The serum T-Protein showed significant decreasing in the specimen of patients as compared with those of the control subjects (p < 0.05). Serum MDA of the patients was found to be significantly (p < 0.05) increased compared with those of the control subjects and decreased after abstinence. SOD, GSH and GPx activities in which were, respectively, 84%, 15%and 44% lower in alcoholic patients than in the control subjects (p < 0.05, respectively), whereas the change of GR and CAT activity were not statistically significant. After 14 days’ abstinence, SOD activity was reduced by 81% (p < 0.05), CAT by 43% (p < 0.05) and GPX by 52% (p < 0.05) in the patients, whereas the change of GSH and GR was not significant. The levels of MDA was significantly correlated with the duration of alcohol dependence (r = 0.538; p <0.001). In addition, the activities of CAT and GSH were significantly correlated with MDA levels (r = 0.701, 0.706 respectively; p < 0.001). In the vitro experiment of the treatment of peripheral blood with ethanol or acetaldehyde, superoxide anion was detected by flowcytometry. The results of present study suggest that oxidative stress occurred and caused subsequently exhaustion of the antioxidants during alcohol dependence.