Studies on the cis-elements in the gene control region of rice sucrose synthase RSus1 gene

• Main Authors: Jui-Che Chang, 張睿哲
• Other Authors: Ai-Yu Wang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/91337436586205514013

碩士 === 國立臺灣大學 === 微生物與生化學研究所 === 92 === Sucrose synthase catalyzes the reversible conversion of sucrose and UDP into fructose and UDPG. The enzyme is encoded by three differentially expressed genes, RSus1, RSus2 and RSus3, in rice. The gene products of RSus1 and RSus2 are ubiquitously present in various rice tissues, while the expression of RSus3 is seed-specific. In this study, we investigated whether the 5’ control region of RSus1 could interact with nuclear proteins from suspension-culture cells of rice (Oryza sativa L. cv. Tainung 67) under different treatments. The results of gel retardation and DNase I footprinting suggest that there are four protein-binding regions between -1117 bp and -750 bp upstream the transcription initiation site of RSus1. Sequence analysis of these four protein-binding regions revealed four distinct elements. When gel retardation experiments were performed with nuclear proteins extracted from cells cultured in the presence of sucrose and cycloheximide, the formation of DNA-protein complexes diminished. The results indicate that binding of proteins to the cis-elements in the gene control region of RSus1 may be sucrose-dependent.