| Summary: | 碩士 === 國立臺灣大學 === 醫學工程學研究所 === 92 === In this study, HepG2 was cultured on several substrates in vitro and was investigated the cell growth, function and morphology with the methods of MTT, LHD, electrophoresis, urea and albumin synthesis analysis and scanning electric microscopy.
In the experiment, HepG2 cell line could attach and spread well on the substrates of EVAL, galactosylated-EVAL, PVDF, Nylon66 and TCPS. However, PVA and chitosan weren’t good substrates for HepG2 to spread and growth. But in the experiment of assaying the quantity of urea and albumin in the culture medium, we found that galactosylated-EVAL and the substrates that cells didn’t grow very well could make the HepG2 cells to secret high quantity of albumin. And the substrates that made cell grow well would decrease the cell function of secreting the albumin. In the experiment of the electrophoresis, cells cultured on the PVA and galactosylated-EVAL substrates with the presence of serum have the higher mobility. But when we removed the serum from the medium, cells cultured on the PVDF and galactosylated-EVAL had the higher mobility relatively. With the addition with cycloheximide, the cells mobility on PVDF and galactosylated-EVAL decreased and was the same with the cell mobility on other substrates. SEM analysis of the HepG2 cultured on chitosan, Nylon66, PVA, galactosylated-EVAL showed the morphology to have the multi-layer structure. And cell morphology cultured on EVAL, PVDF and TCPS showed to be mono-layer structure.
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