Development of A Model System for Evaluation of Biomaterials in Dentistry

• Main Authors: Yi Ho, 何易
• Other Authors: Juo-Song Wang
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/13262517876440084721

碩士 === 國立臺灣大學 === 臨床牙醫學研究所 === 92 === Biological evaluation of dental material is performed to determine the potential toxicity resulting from contact of the component materials with the body. Evaluation of any new material for oral use requires data from systematic testing to ensure that the benefits provided by the use of the materials will exceed any potential risks produced by the materials. Continuous cell lines, like L939 or 3T3 mouse fibroblasts, are being routinely used for the testing of cytotoxic properties of dental materials because their reproducible growth rates and biologic responses. Nevertheless, the question whether these are adequate target cells has often been raised with regard of the clinical relevance of data derived from in vitro studies. Most dental materials came to contact with mucosal cells, therefore, a stable cell line that is closer to normal epithelial cells is being pursuit for evaluation of these materials. In this report two native SCC (Squamous Cell Carcinoma) cell lines were developed - TW 1.5 and TW 2.6, from primary culture of squamous cell carcinoma of tongue and squamous cell carcinoma of buccal mucosa specimens, respectively. The cell line TW1.5 was immortalized through spontaneous mutation, and the cell line TW2.6 was immortalized through in vivo selection in skid mice. Characterization of these two cell lines including morphology, cell cycle regulation protein expression, growth curve, sequencing o fp53 gene, and chromosome analysis had been carried out in vitro. These two cell lines are native, keratinized, and may have characters that are similar to normal epithelium cells of mucosa. These SCC cell lines might be a good candidate for material testing because of their cellular origin and growth potential. They may also be useful for researching the possible mechanism of carcinogenesis in SCC patients and development of new cancer therapeutic strategies.