Analysis of random amplified polymorphic DNA (RAPD) markers associated with yield traits of tomato under heat stress

• Main Authors: Shao-Pei Lee, 李紹珮
• Other Authors: Hsiao-Feng Lo, Ph.D.
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/82955925450323541644

碩士 === 中國文化大學 === 生物科技研究所 === 92 === Tomato contains many kinds of chemical constituents, such as Vitamin C and lycopene, which are considered as free radicals scavenger and thus protects people from some chronic disorders or reduces the rate of some cancers. Most tomato varieties are adaptive to warm and dry weather, and don’t show heat tolerance. The optimum temperature for growing tomato is 15-20/20-26 °C night/ day. During summer time in Taiwan, the production of tomato is decreased due to high temperature and high rainfall. Heat tolerance of tomato is a quantitatively inherited trait and easily affected by environmental conditions. A total of 43 F7 recombinant inbred lines derived from a heat tolerant CL 5915 crossed with a heat sensitive L4422 (L. Pimpinellifolium) were grown in the screenhouse at PCCU in the summer of 2002. Five heat tolerance-related traits were scored: flower number, fruit number, fruit set, fruit weight and fruit yield. RAPD analysis was used to identify any primer associated with the flower number, fruit number and fruit weight by bulking DNAs of skewed lines (bulked segregant analysis). A total of 200 random primers were screened. Only 14 RAPD-PCR products generated by OPERON primer with DNA template from L4422, CL5915 and F7 RILs (Recombinant inbred line) were found with polymorphism. Among them, 88 bands were amplified, in which 23 bands showed informative and were used for χ2 test. An average of 1.6 bands out of 6.3 bands was generated by a primer and thought to be informative. Combination of the mentioned 5 traits and the informative markers, we were able to identify and locate the RAPD markers linked to the QTL of traits using Mapmaker/EXP 3.0b and Mapmaker/QTL 1.1b softwares. Two linkage groups were constructed from Mapmaker: C09 and S13; K06, K14, and P08. Nevertheless, all these 5 primers didn’t show any linkage to the five heat tolerance-related traits.