| Summary: | 碩士 === 靜宜大學 === 應用化學研究所 === 92 === Three types of dry liquid natto powders, 24hr-liquid natto, 48hr-liquid natto and 96hr-liquid natto, were obtained from the fermentation of Tainan No.2 soybeans by using Bacillus natto in a period of 24hr, 48hr, and 96hr, respectively, filtration, centrifugation and freez-drying. Among them, the yield of 96hr-liquid natto was the highest.
The results of effective ingredient and various cosmetic analyses for those three types of liquid natto are summaried as follows：
1)They all possess the high promotion of Dopachrome formation based on the Dopachrome inhibition tests；particularly, 96hr-liquid natto has the most effectiveness.
2)They all can absorb the more UV-A and UV-B light in the concentration of 100.0 mg/ml compared to those of commercial sunscreen products such as Parsol 1789, Benzophenone-3, and octyl methoxycinnamate in the concentration of 0.5 mg/ml；especially, 96hr-liquid natto has the most effectiveness.
3)They all have the excellent moisture maintenance to decrease the dehydration of skin, and to increase the hydrated level of stratum corneum.
4)They all have the antioxidant activity based on the DPPH radical scavenging test, the reducing power determination, and the chelation of Fe2＋ ion. Among them, 96hr-liquid natto has the highest effectiveness.
5)They all can inhibit the growth of Propionibacterium ance slightly；especially, 24hr-liquid natto has the most effectiveness.
6)They all contain polyphenols, flavonoids, and anthocyanins；especially, 96hr-liquid natto possesses the hightest amount of those three components.
7)They all demonstrated the high stability while 1.0% liquid natto was mixed with lotion, cream, and mask, respectively, based on the cosmetic stability tests such as high temperature, low temperature, room temperature, circulating temperature, and UV light irradiation .The stability tests can maintain over five months.
Therefore, readily prepared liquid natto can be employed to the skin care products since they have various functions such as UV-A and UV-B absorption, antioxidant activity, antibacterial activity for Propionibacterium ance, and high moisture maintenance. Particularly, 96hr-liquid natto was the most valuable candidate on the skin care.
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