Secretion of Lipopolysaccharide Induced Interleukin-6 Expression by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on Human Astrocytoma U373 MG Cells

• Main Authors: Hui-Wen Chang, 張慧文
• Other Authors: Yi-Yuan Yang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/25073968916899338973

碩士 === 臺北醫學大學 === 生物醫學技術研究所 === 92 === Abstract Suppression of Lipopolysaccharide Induced Interleukin-6 Secretion by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on Human Astrocytoma U373 MG Cells Hui-Wen Chang Thesis advised by: Yi-Yuan Yang, Ph.D. The effect of environmental pollutants on the immune system has been a well-studied and controversial topic, with dioxins heading the list of molecules under investigation. Mainly produced as a by-product of the manufacture of materials requiring the use of chlorinated phenol, or as a result of the incineration of chlorinated chemical products. Dioxin is suspected to cause adverse effects on the development of the central nervous system (CNS). Astrocytes are now believed to play important roles in repairing injured neurons. It is now recognized that inflammation is centrally involved in the pathogenesis of a number of neurodegenerative diseases. AIDS, dementia, and Alzheimer’s disease lead to an increase in the level of IL-6 (interleukine-6) in the CSF and brain. In this study, we investigated the effects of TCDD (2,3.7.8-Tetrachlorodibenzo-p-dioxin) on IL-6 expression in U373 MG cells. Data indicated that TCDD doesn’t effect of IL-6 secretion on human Astrocytoma U373 MG cells, but suppress of LPS (lipopolysaccharide) -induced IL-6 secretion as measured by enzyme-linked immunosorbent assay (ELISA). The treat effects of TCDD were not due to decreased U373 MG cells viability as assessed by MTT、LDH test. No effect of ARNT expression was estimated through immunocytochemistry and western blot in U373 MG Cells by TCDD. Furthermore, TCDD inhibit AhR, IL-1β and IL-6 mRNA expression on rat cortical neuron as detected by RT-PCR. But TCDD uptake resulted in a significant increase in IL-6 level in the mouse CSF. These results suggest that in vivo exposure to TCDD may differ between tissues. Clearly, the effects of TCDD in vivo are varied and complex, likely involving interactions between many cell types and cytokine feedback loops.