The Effects of Soy Isoflavones ( Genistein and Daidzein) on MC3T3-E1 Cells Proliferation and Bone Protein Expression
碩士 === 臺北醫學大學 === 保健營養學系 === 92 === The proliferation effect of isoflavones(genistein and daidzein) were investigated using an MTS assay and DNA content on MC3T3-E1 cells. The samples were tested at levels of 10-9 to 10-5M of isoflavones for 24, 48, 72 and 96 hr incubation. The results obtained from...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2004
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| Online Access: | http://ndltd.ncl.edu.tw/handle/04130240365386244856 |
| Summary: | 碩士 === 臺北醫學大學 === 保健營養學系 === 92 === The proliferation effect of isoflavones(genistein and daidzein) were investigated using an MTS assay and DNA content on MC3T3-E1 cells. The samples were tested at levels of 10-9 to 10-5M of isoflavones for 24, 48, 72 and 96 hr incubation. The results obtained from the two assys were consisted and also exhibited dose-dependent effects except 10-5M. At the level of 10-8 and 10-7M, isoflavones had the best result for cell proliferation. Genistein and daidzein stimulated an increase in alkaline phosphatase(ALP) activity on MC3T3-E1 cells in a dose-dependent manner(10-9 ~10-7M). Isoflavones treatment(10-9 ~10-7M) significantly increase the production of osteopontin(OPN) and Type I collagen protein, but not on OPN mRNA expression. In summary: isoflavones at dietary achievable level(10-9 ~10-7M) stimulated the proliferation and DNA content of MC3T3-E1 cells. Isoflavones also increased bone marker synthesis(ALP, OPN and Type I collagen) and could be beneficial for bone growth.
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