Artificial skin development: preliminary studies of a composite matrix

• Main Authors: Ting-Wei Lin, 林庭瑋
• Other Authors: TSUIMIN TSAI
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/16098742280966385285

碩士 === 臺北醫學大學 === 生物醫學材料研究所 === 92 === So far, the most established system in tissue engineering is the development of the artificial skin. In order to reduce the number of animals used in trials, an in vitro model would be helpful. In this study, the idea is to create a skin substitute that could mimic the real skin by first develop a composite matrix for cell and tissue culture. There are four parts in this plan. First, obtain the type I collagen from porcine skin. Second, prepare a 3-D, sponge-liked (porous) collagen based matrix with chitosan and chondroitin sulfate. Third, investigate the physicochemical properties of the prepared material by Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared (FTIR) spectroscopic studies, Dynamic Mechanical Analysis (DMA) and Scanning Electron Microscopic (SEM) studies. Fourth, culture the human dermal fibroblasts and epidermal keratinocytes into the matrix and to observe the cell growth and proliferation by SEM and H & E stain.