Artificial skin development: preliminary studies of a composite matrix
碩士 === 臺北醫學大學 === 生物醫學材料研究所 === 92 === So far, the most established system in tissue engineering is the development of the artificial skin. In order to reduce the number of animals used in trials, an in vitro model would be helpful. In this study, the idea is to create a skin substitute that could m...
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ndltd-TW-092TMC006840052016-06-15T04:17:07Z http://ndltd.ncl.edu.tw/handle/16098742280966385285 Artificial skin development: preliminary studies of a composite matrix 膠原蛋白/幾丁聚醣/硫酸軟骨素做為人工皮膚基材之研發 Ting-Wei Lin 林庭瑋 碩士 臺北醫學大學 生物醫學材料研究所 92 So far, the most established system in tissue engineering is the development of the artificial skin. In order to reduce the number of animals used in trials, an in vitro model would be helpful. In this study, the idea is to create a skin substitute that could mimic the real skin by first develop a composite matrix for cell and tissue culture. There are four parts in this plan. First, obtain the type I collagen from porcine skin. Second, prepare a 3-D, sponge-liked (porous) collagen based matrix with chitosan and chondroitin sulfate. Third, investigate the physicochemical properties of the prepared material by Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared (FTIR) spectroscopic studies, Dynamic Mechanical Analysis (DMA) and Scanning Electron Microscopic (SEM) studies. Fourth, culture the human dermal fibroblasts and epidermal keratinocytes into the matrix and to observe the cell growth and proliferation by SEM and H & E stain. TSUIMIN TSAI 蔡翠敏 2004 學位論文 ; thesis 0 zh-TW |
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碩士 === 臺北醫學大學 === 生物醫學材料研究所 === 92 === So far, the most established system in tissue engineering is the development of the artificial skin. In order to reduce the number of animals used in trials, an in vitro model would be helpful. In this study, the idea is to create a skin substitute that could mimic the real skin by first develop a composite matrix for cell and tissue culture. There are four parts in this plan. First, obtain the type I collagen from porcine skin. Second, prepare a 3-D, sponge-liked (porous) collagen based matrix with chitosan and chondroitin sulfate. Third, investigate the physicochemical properties of the prepared material by Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared (FTIR) spectroscopic studies, Dynamic Mechanical Analysis (DMA) and Scanning Electron Microscopic (SEM) studies. Fourth, culture the human dermal fibroblasts and epidermal keratinocytes into the matrix and to observe the cell growth and proliferation by SEM and H & E stain.
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TSUIMIN TSAI |
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TSUIMIN TSAI Ting-Wei Lin 林庭瑋 |
author |
Ting-Wei Lin 林庭瑋 |
spellingShingle |
Ting-Wei Lin 林庭瑋 Artificial skin development: preliminary studies of a composite matrix |
author_sort |
Ting-Wei Lin |
title |
Artificial skin development: preliminary studies of a composite matrix |
title_short |
Artificial skin development: preliminary studies of a composite matrix |
title_full |
Artificial skin development: preliminary studies of a composite matrix |
title_fullStr |
Artificial skin development: preliminary studies of a composite matrix |
title_full_unstemmed |
Artificial skin development: preliminary studies of a composite matrix |
title_sort |
artificial skin development: preliminary studies of a composite matrix |
publishDate |
2004 |
url |
http://ndltd.ncl.edu.tw/handle/16098742280966385285 |
work_keys_str_mv |
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