| Summary: | 碩士 === 臺北巿立體育學院 === 運動科學研究所 === 92 === ABSTRACT
Intense exercises can cause accretion of reactive oxygen species (ROS) with relative depletion of antioxidants, resulting in oxidative stress. It thus is important to study whether if different types of sports will cause oxidative stress. A rugby tournament is an intensively intermissive exercise. The purpose of this study was to investigate the changes of reactive oxygen species and antioxidants in leukocytes subpopulation (PMN, MON, LYM) after one rugby game. Blood samples were taken at several time points: prior to the first quarter, after the fourth quarter, and 72 hours after the game. Concentrations of intracellular hydrogen peroxide (H2O2) and glutathione (GSH) were measured by FACS Calibur Flow Cytometry. Results indicated concentrations of hydrogen peroxide in three leukocyte subtypes were significantly decreased and maintained at low level after the game of 72 hours. However, the concentrations of glutathione in these leukocyte subtypes were dramatically increased after the fourth quarter, but they were decreased after the game of 72 hours. A consistent value of GSH concentration whether it was above or below the average value could not be obtained. Nevertheless, GSH concentration of lymphocyte (LYM) after the game of 72 hours had the tendency to rapidly decline. Its relative declined degree was the highest in three leukocyte subtypes. This research may conclude that after one rugby game, the concentrations of three leukocyte subtypes have similar changes of reactive oxygen species and antioxidants. However, a diversity of changing degree is observed in these leukocyte subtypes. To sum up, after one rugby game, the concentrations of antioxidants accretion with relative depletion of reactive oxygen species. These two factors won’t result in oxidative stress.
Key words: Rugby, Oxidative Stress, Leukocytes
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