Cloning and characterization of a novel RNA-binding

• Main Authors: Ying-Shing Huang, 黃盈馨
• Other Authors: Jim C. Fong
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/32703260449683159990

碩士 === 國立陽明大學 === 生物化學研究所 === 92 === In 3T3-L1 adipocyte, under treatments of glucose, isoproterenol, ET-1, ET-1+Iso, dexamethasone, TNF-α, many genes were up-regulated or down-regulated. By the method of subtractive suppression hybridization (SSH), we found 561 genes were influenced by treatment as mentioned above. Thus these genes might play important roles in the regulation of cell function. Using bioinformatics, we examined these genes, and we focused on one gene, which accession number in NCBI is XM_128090. XM_128090 is up-regulated by isoproterenol. The gene product is XP_128090 and the function of this protein is still unknown. Analyzing the protein sequence of XP_128090, two important conserved domains were found. These domains are LA domain and RRM (RNA recognition motif). Using BLAST (basic local alignment search tool) to compare XM_128090 sequence in a sequence database, we found homologues genes in many species, including human. The result showed that various mouse tissues contain this gene. Human MCF-7 cells also contain the homologues gene. Due to the requirement of energy to feed uncontrolled proliferation, cancer cells strongly rely on glucose as energy source. Inhibition of glucose metabolism sensitizes tumor cells for apoptosis. Our result indicated that the LA-RRM binding protein might inhibit glucose transporter-1 expression in MCF-7 cells. Since glucose uptake into MCF-7 cells is mediated mainly by GLUT-1, we speculate that this protein might be involved in the regulation of growth of breast tumor cells.