Investigation of the Mechanisms Underlying Aristolochic Acid-Induced Kidney Injury

• Main Authors: Jiun-Liang Chen, 陳俊良
• Other Authors: Jong-Hwei Su Pang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/45718352810064632742

碩士 === 長庚大學 === 傳統中國醫學研究所 === 93 === Objective: To investigate the mechanism underlying aristolochic acid-induced kidney injury. Both cell model and animal model experiments were carried out to study the in vitro and in vivo effects of aristolochic acid. Methods: In vitro study:Renal tubular epithelial cells ( NRK-52E ) were cultured in 95 % Dulbecco's modified Eagle's medium (DMEM) with 0.1mM non-essential amino acid and 5% calf serum. The cells viability was measured by theMTT(3-[4,5-Dimethylthiazol-2-yl]-2,5-dihenyltetrazolium bromide；Thiazolyl blue) assay. Apoptotic cells were identified by propidium iodide ( PI ) stain, TUNEL(TdT-mediated dUTP nick end labelling) assay and Annexine-V FITC (Fluoreszeinthiocyanat ) stain. RNA expression was analyzed by RT-PCR. In vivo study: The histopathology of FVB mice was examed by hemotoxylin and eosin stain, TUNEL assay and immunohistochemical method. Results: (1)Aristolochic acid in the concentration above 10μg/ml could significantly reduce the viability of NRK-52E rat kidney proximal renal tubule cell. (2) TUNEL stain demonstrated the apoptosis of renal tubule cells induced by aristolochic acid. (3) Annexine-V FITC (Fluoreszeinthiocyanat ) stain further confirmed the apoptosis of renal tubule cells induced by aristolochic acid.(4) Aristolochic acid in the concentration above 10μg/ml could increase the expression of Bax mRNA.(5)Pretreatment of cyclosporin A (7.5μg/ml ) could reduce the apoptosis of renal tubule cells induced by aristolochic acid.(6)Radix salviae miltiorrhizae could decrease the loss of cells by aristolochic acid. was significantly inhibited. Pretreatment with radix salviae miltiorrhizae could prevent the cell apoptosis of kidney proximal renal tubule cells induced by aristolochic acid.(7)The FVB mice model of aristolochic acid nephropathy confirmed the cell apoptosis of kidney proximal renal tubule cells. Conclusions : Aristolochic acid in the concentration above 10μg/ml could significantly reduce the viability of NRK-52E rat kidney proximal renal tubule cell and increase the expression of Bax mRNA via mitochondria pathway to induce the apoptosis of renal tubule cells. Radix salviae miltiorrhizae exerts dose-dependent protective effect on the aristolochic acid-induced renal tubular epithelial cells injury by preventing the apoptosis of renal tubular epithelial cells induced by aristolochic acid. The animal model of aristolochic acid nephropathy confirmed the apoptosis of renal tubular epithelial cells induced by aristolochic acid by TUNEL stain. We have set up the FVB mice model of Chinese herb-induced tubular interstitial nephritis which could help us to screen and study the Traditional Chinese medicines in the prevention or treatment of Chinese herb-induced tubular interstitial nephritis and the early renal fibrosis.