| Summary: | 碩士 === 長庚大學 === 基礎醫學研究所 === 93 === Human myelogenous leukemia K562 cells were induced to undergo erythroid differentiation by treatment with hydroxyurea (HU). However, the cellular mechanism by which HU exert its effects on tumor cells, leading to inhibition of cell growth and induction of differentiation markers, are largely unknown. This study examined (1) the role of different mitogen-activated protein kinase signal transduction pathways in HU-induced erythroid differentiation of K562 cells. (2) The mechanism of down regulation of nucleophosmin (NPM)/ B23 promoter during HU- induced erythroid differentiation. (3) To study the biological role of B23 in K562 cell differentiation induced by HU. Here, we show that the relationship between NPM / B23 and interferon regulatory factor- 1 (IRF-1), mediates down-stream target gene activation that during HU-induced erythroid differentiation of K562 cells. Thus, this study indicate effects of HU on down regulation of NPM/ B23 may be caused by inhibition of histone deacetylase 1(HDAC 1).
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