Development of in vitro Diagnostic Kits for Colon Cancer and Nasopharyngeal Carcinoma with Specific Panel of Antibodies

• Main Authors: Yu-Han chen, 陳鈺翰
• Other Authors: 詹爾昌
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/00179742206173405886

碩士 === 長庚大學 === 醫學生物技術研究所 === 93 === Colon cancer has been listed as the third cause of death among cancers in Taiwan. However, the lack of an effective early detection screening methods is the major barrier to improve the treatment of patients with colon cancer. According to recent studies, some tumor-associated antigens can specifically recognize cancer patient’s autologous antibodies. Therefore, we used nine colon cancer antigens to detect specific antibodies in patient’s serum. Antibody reactivity against these antigens were determined in sera with from patients with colon cancer (n=105) and healthy donors (n=54). The five antigens which are named NY-CO-8, NY-CO-9, NY-CO-13, NY-CO-16 and NY-CO-20 got better reactivities, and sensitivity of these antigens was in the range of 17.1%- 36.8%. The combined analysis of the panel of the antibody reactivities, the sensitivity is 61.0 %, the specificity is 92.6 %. The addition analysis of CEA value could increase the sensitivity to 70.1% and, 43.5 % of the patients at early stages are detected. The ELISA format is cost-effective and feasible for mass screening; the specific panel of these profiles may be worth for early detection.