Pharmaceutical Studies of San-Huang-Shel-Shin-Tang
碩士 === 中國醫藥大學 === 中國藥學研究所 === 93 === San-Huang-Shel-Shin-Tang (SHSST) is a popular traditional Chinese medicine formula, including Da Huang (Rhei Rhizoma), Huang Lien (Coptidis Rhizoma) and Huang Qin (Scutellariae Radix). In this study, the constituents (coptisine, palmatine, berberine, baicalin, ba...
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ndltd-TW-093CMCH00490052015-10-13T12:56:39Z http://ndltd.ncl.edu.tw/handle/02811851483050461317 Pharmaceutical Studies of San-Huang-Shel-Shin-Tang 三黃瀉心湯之生物藥學研究 Pei-Hsun Hsieh 謝佩勳 碩士 中國醫藥大學 中國藥學研究所 93 San-Huang-Shel-Shin-Tang (SHSST) is a popular traditional Chinese medicine formula, including Da Huang (Rhei Rhizoma), Huang Lien (Coptidis Rhizoma) and Huang Qin (Scutellariae Radix). In this study, the constituents (coptisine, palmatine, berberine, baicalin, baicalein, wogonin, emodin, aloe-emodin, rhein, chrysophanol) of SHSST extract were quantified by HPLC method. By comparing blood levels in rats and urinary recovery in humans, we attempted to evaluate the bioavailibility and bioequivalence between decoction and commercial extract of SHSST. The results showed that the contents of the bioactive constituents in commercial extract of SHSST among manufactures were quite different. The aglycones increase remarkably after hydrolyzed with b-glucosidase, indicating that polyphenols of SHSST exist mainly as glycosides. Rats were given traditional decoction and commercial extract of SHSST containing comparable dose of rhein, alkaloids were not detected in serum. Flavonoids and anthraquinones were found predominantly as sulfates and glucuronides in the bloodstream. Beside baicalein, the AUC/dose and Cmax/dose of sulfates/glucuronides of aloe-emodin, wogonin, rhein, emodin and chrysophanol after intake of commercial extract were higher than those from decoction. All consitituents of SHSST in these two dosage forms were not bioeqivalent. After ingesting SHSST commercial extract, the urinary recoveries of rhein and emodin sulfates/glucuronides were higher than decoction. Other four constituents were recovered more after ingesting decoction. The polyphenols of SHSST in these two dosage forms were essentially not bioeqivalent. All constituents demonstrated enterohepatic circulation. The excretion half-life of all constituents ranged from 2.5 to 14.8 h and the variability among individuals was large. In vitro study showed that emodin, aloe-emodin, baicalin and their conjugated metabolites inhibited AAPH-induced hemolysis in a concentration-dependent manner. Moreover, the serum obtained from rats fed SHSST significantly decreased the hemolysis. Furthermore, equilibrium dialysis method was used to evaluate protein binding of the metabolites of SHSST and the results showed they were very highly bound and ranged between 83.0% ~98.6%. Pei-Dawn Lee Chao Su-Lan Hsiu Yu-Chi Hou 李珮端 徐素蘭 侯鈺琪 2005 學位論文 ; thesis 185 zh-TW |
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碩士 === 中國醫藥大學 === 中國藥學研究所 === 93 === San-Huang-Shel-Shin-Tang (SHSST) is a popular traditional Chinese medicine formula, including Da Huang (Rhei Rhizoma), Huang Lien (Coptidis Rhizoma) and Huang Qin (Scutellariae Radix). In this study, the constituents (coptisine, palmatine, berberine, baicalin, baicalein, wogonin, emodin, aloe-emodin, rhein, chrysophanol) of SHSST extract were quantified by HPLC method. By comparing blood levels in rats and urinary recovery in humans, we attempted to evaluate the bioavailibility and bioequivalence between decoction and commercial extract of SHSST.
The results showed that the contents of the bioactive constituents in commercial extract of SHSST among manufactures were quite different. The aglycones increase remarkably after hydrolyzed with b-glucosidase, indicating that polyphenols of SHSST exist mainly as glycosides. Rats were given traditional decoction and commercial extract of SHSST containing comparable dose of rhein, alkaloids were not detected in serum. Flavonoids and anthraquinones were found predominantly as sulfates and glucuronides in the bloodstream. Beside baicalein, the AUC/dose and Cmax/dose of sulfates/glucuronides of aloe-emodin, wogonin, rhein, emodin and chrysophanol after intake of commercial extract were higher than those from decoction. All consitituents of SHSST in these two dosage forms were not bioeqivalent.
After ingesting SHSST commercial extract, the urinary recoveries of rhein and emodin sulfates/glucuronides were higher than decoction. Other four constituents were recovered more after ingesting decoction. The polyphenols of SHSST in these two dosage forms were essentially not bioeqivalent. All constituents demonstrated enterohepatic circulation. The excretion half-life of all constituents ranged from 2.5 to 14.8 h and the variability among individuals was large.
In vitro study showed that emodin, aloe-emodin, baicalin and their conjugated metabolites inhibited AAPH-induced hemolysis in a concentration-dependent manner. Moreover, the serum obtained from rats fed SHSST significantly decreased the hemolysis. Furthermore, equilibrium dialysis method was used to evaluate protein binding of the metabolites of SHSST and the results showed they were very highly bound and ranged between 83.0% ~98.6%.
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author2 |
Pei-Dawn Lee Chao |
author_facet |
Pei-Dawn Lee Chao Pei-Hsun Hsieh 謝佩勳 |
author |
Pei-Hsun Hsieh 謝佩勳 |
spellingShingle |
Pei-Hsun Hsieh 謝佩勳 Pharmaceutical Studies of San-Huang-Shel-Shin-Tang |
author_sort |
Pei-Hsun Hsieh |
title |
Pharmaceutical Studies of San-Huang-Shel-Shin-Tang |
title_short |
Pharmaceutical Studies of San-Huang-Shel-Shin-Tang |
title_full |
Pharmaceutical Studies of San-Huang-Shel-Shin-Tang |
title_fullStr |
Pharmaceutical Studies of San-Huang-Shel-Shin-Tang |
title_full_unstemmed |
Pharmaceutical Studies of San-Huang-Shel-Shin-Tang |
title_sort |
pharmaceutical studies of san-huang-shel-shin-tang |
publishDate |
2005 |
url |
http://ndltd.ncl.edu.tw/handle/02811851483050461317 |
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