The immunomodulatory effect of you- gui- wan on repetitive Dermatogoides pteronyssinus challenged asthmatic mice model

• Main Authors: Chih-Che Lin, 林志哲
• Other Authors: Shung-Te Kao
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/74577708241611195109

碩士 === 中國醫藥大學 === 中國醫學研究所 === 93 === The immunomodulatroy effect of you- gui- wan on repetitive Dermatogoides pteronyssinus challenged asthmatic mice model Chih-Che Lin Major professor: Shung-Te Kao Institute of Chinese Medical Science, China Medical University Allergic asthma is a chronic airway inflammatory disease, which is characterized by the Th2-bios immune response, eosinophil infiltration, mast cell activation, epithelial hypertrophy, goblet cell hyperplasia, airway hyperresponsiveness, and excessive mucus secretion in airway. Dermatogoides pteronyssinus（Der p）is one of the most prominent and important species of house dust mite in allergic asthma in Taiwan. We used Der p to induce BABL/c mice as asthmatic animal model which help us to understand the therapeutic mechanism of you- gui- wan（YGW）. In this study, we found that YGW could inhibit the recruitment of monocyte and eosinophil in brochoalveolar lavage fluid （BALF）. Simultaneously, it could reduce CD3＋CD4＋and CD4＋CD25＋ T cell in lymphacyte ratio significantly. YGW also could down-regulated IL-5 in serum to inhibit eosinophil infiltration and up-regulated IL-12 in BALF to change Th2-bios. Otherwise, EMSA and immunohistochemistry stain demonstrated YGW could inhibit NF-κB expression in lung. Collagen assay and hematoxylin-eosin stain indicated YGW could reduce airway remodling in airway. In RT-PCR experiment, we also found that YGW could inhibit TGF-β1, IL-10, IL-13, eotaxin, RANTES, and MCP-1 mRNA expression which demonstrated that the major mechanism of YGW still inhibited late asthmatic response. According to above results, it is reasonable that use kidney-yang drug such as YGW to treat asthma at relapsed phase.