| Summary: | 碩士 === 中國醫藥大學 === 中國醫學研究所 === 93 === The involvement of serotonin in Suanzaorentang-induced sleep regulation
Chun-Pang Lin
Major professor：Fang-Chia Chang, Ph.D.
Institute of Chinese Medical Science, China Medical University, Taiwan, ROC
ABSTRACT
Suanzaorentang (SZR Tang) is a compound of traditional Chinese medicine, which is widely used for treatment in insomnia. It was first demonstrated in the “Jin Kui Yao Lve”written by Chong-Jing Chang. In this study, we investigated the hypnotic effect of SZR Tang in rats. Furthermore, we examined the role of serotonin (5-hydroxytryptamine, 5-HT) in SZR Tang-induced sleep alterations by microinjection of 5-HT receptor antagonists into the lateral ventricle of the rat. We also drew out anyone of the ingredients from SZR Tang to determine whether withdrawing anyone ingredient from Suanzaorentang affects its hypnotic action.
Our results indicate that the high dose (4.0 g/kg) of SZR Tang increased the NREM sleep to from 16.79±1.87 % obtained after baseline to 33.02±2.35 %. However, starch placebo (4.0 g/kg) also increased NREMS to 25.12±2.15 % . REM sleep has no change apparently after both placebo and SZR Tang. All three subtypes of 5-HT antagonists decreased SZR Tang-induced increase in NREM sleep. In addition, our results also demonstrate that no statistical change in both NREM sleep and REM sleep comparing with those from baseline recording when one ingredient was withdrew.
These results suggest that the SZR Tang exhibits the hypnotic effect in rats. The SZR Tang-induced increase in NREM sleep was block by the 5-HT antagonists, suggesting the involvement of serotonin in this mechanism.
When anyone of the ingredient withdraws from SRZ Tang, it exhibits no change in both NREM sleep and REM sleep. This indicates that the total five ingredients of SZR Tang possess the hypnotic effect rather than the prescription lacking of anyone ingredient.
Keywords：Suanzaorentang, serotonin, sleep.
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