Exposuer Assessment of Furfural-Validation of the Sampling and Analysis Mwthod for Furfural in Air amd Food

• Main Authors: Kao Kuo-Yuan, 高國源
• Other Authors: Tsai Shih-Wei
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/82273078412873428696

碩士 === 中國醫藥大學 === 環境醫學研究所 === 93 === The aim of this research was to develop a new sampling and analysis method for furfural in air and food based on the technique of solid-phase microextraction(SPME) and the use of O-2,3,4,5,6-(pentafluoro benzyl)hydroxylamine(PFBHA). For air sample, the polydimethylsiloxane/divinylvenzene (PDMS/DVB) fiber was used for sampling. PFBHA was first coated onto the fiber by headspace extraction and the SPME diffusive sampler was then assembled. The sample was analyzed directly by GC/MS for thermal desorption and quantification. The sampling rate of the passive sampler was determined and effects from different environmental conditions were investigated. Besides, the side-by-side comparison with the OSHA Organic Method#72 were also performed. For food sample, the PDMS/DVB fiber was also used. PFBHA was first coated onto the fiber then the headspace extraction for furfural in sample was performed. After sampling, the sample was analyzed directly by GC/MS for thermal desorption and quantification. Factors that might affect the extraction efficiencies were evaluated. For air sample, the results showed that different relative humidities (6%~80%) and different concentrations (3.93~125.76mg/m3) will not affect the performance of the sampler. However, the sampling rates were diverse at different temperatures (The sampling rates were (3.758±0.062)×10-2, (5.023±0.102)×10-2, and (13.254±0.112)×10-2 cm3/min at 4C, 25C and 35C, respectively. On the other hand, it was shown that the results between the SPME device and the OSHA Organic Method#72 were liner and consistent (r=0.9817, slope=1.054±0.055). Detection limit was 0.565 ng. For food sample, it was found that extraction time, temperature, and ionic strength will affect the efficiency of extraction. The extraction conditions chosen for vinegar and beer were: temperature at 20C, concentration of NaCl spiked equal 142 mg/mL and 10 min for extraction. Conditions for infant formulas were: temperature at 20C, concentration of NaCl spiked equal 71 mg/mL and 15 min for extraction. Detection limits were 3.087 μg/L(vinegar), 8.237 μg/L(beer), and 14.047 μg/L(infant formulas).