PROTEOMIC APPROACHES TO IDENTIFY HEPATITIS C VIRUS NS5B RNA POLYMERASE-INTERACTING PROTEINS

• Main Authors: Jing-Tang Huang, 黃景堂
• Other Authors: Ju-Chien Cheng
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/64850437781298980141

碩士 === 中國醫藥大學 === 醫學研究所 === 93 === Abstract Hepatitis C virus (HCV) nonstructure protein 5B (NS5B) is a RNA-dependent RNA polymerase that acts as a key player in the HCV replication complex. Some viral and cellular factors were reported to involve in HCV replication. However, the components of the HCV replication complex are still not yet completely understood. In this study, the HCV NS5B was used as the bait in a pull down assay to screen for NS5B-interacting proteins present in Huh 7 hepatoma cells. After mass spectrophotometric analysis, a putative lipogenic enzyme, fatty acid synthase (FAS), was identified to interact with NS5B. Co-immunoprecipitation analysis further confirmed the direct binding between the cellular protein and HCV NS5B. In addition, the expression of viral proteins and RNA in HCV subgenome replicon cells was decreased by treatment with C75, a specific FAS inhibitor. Together, these results suggest a critical role of FAS in the regulation of HCV infection through the modulation of HCV replication complex.