| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 93 === Atherosclerosis is the major reason to cause these diseases. Based on the most acceptable theory is endothelial dysfunction represented as migration and proliferation in smooth muscle cell, the efficacious inhibition of migration and proliferation in smooth muscle cell is the major direction to preventing atherosclerosis. Traditionally, Nuciferine leaf was reported to possess powerful effect on decreasing serum lipids. It means that Nuciferine leaf also possess the same effect on preventing atherosclerosis. But upon to now, the researchers just emphasized the effects of Nuciferine leaf in clinical research but not in the mechanisms on preventing atherosclerosis. Because of that, we designed a series of experiments to study how Nuciferine leaf prevents atherosclerosis, and what were the mechanisms. The experimental results showed that Nuciferine leaf extract has efficacious inhibition on LDL- preoxidation-relative electrophoretic mobility, Apo B fragmentation, TBARS formation and diene formation. we used flow cytometry assay, MTT assay, DNA fragment assay and the result showed that Nuciferine leaf extract could induced apoptosis of the proliferated smooth muscle cell.and activated the caspase cascade and triggered apoptosis. Further wound healing, gelatin zymography and Boyden chamber study found that Nuciferine leaf extract could inhibited smooth muscle cell migration. According to the above, we suggested that Nuciferine leaf possessed the ability to reduce atherosclerosis via inhibiting the endothelial dysfunction. Furthermore, it had the potential to develop to a new health-caring herb to prevent atherosclerosis.
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