| Summary: | 碩士 === 中原大學 === 機械工程研究所 === 93 === For the traditional immobilized metal affinity chromatography (IMAC), the nickel is used to modify the surface of the beads in chromatographic column for capturing the histidines (his)-tagged protein to make up a rapid purification system. Based on IMAC, a nickel coated protein chip was developed by electroplating technology and its capture property was tested by the immunoassay with a high sensitivity of fluorescence confocal scanner. Taguchi method was adopted to determine the optimal process of protein chip. Hence, a series of L18 experiments by Orthogonal Array rule were performed. The major purpose of our research is the planarization of IMAC technology so that bio-particles can be absorbed on protein chip for further analysis. The advantages of this novel protein chip are good performance, high repeatability, and less cost.
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