Studies on the antioxidant component and bioactivities activities of Osmanthus fragran Lour. in Taiwan.

• Main Authors: Kuo Fang-Jung, 郭芳榕
• Other Authors: Hung Chien-Ya
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/62158527270201642647

碩士 === 中華醫事學院 === 生物科技研究所 === 92 === This study evaluates the antioxidant activities and toxicity of compounds isolated from Osmanthus fragrans flower extract. Chapter 2 was focused on extraction and identification of antioxidative components from O. fragrans flower. The methanol extract of O. fragrans flower that have total polyphenol 123.2 mg /g extract and 94.5 % scavenging effect on DPPH radical was better among the 11 flower-herbs. O. fragrans flower was extracted with 65℃ methanol, and then fractionated with the different polarities. The result showed that the antioxidant activity with the descending order was : methanol extract ≧ chloroform fraction = butanol fraction > water fraction. The chloroform fraction was separated and identified to be verbascoside (1), rutin (2), ligustroside (3), phillygenin (4), p-hydroxyphenylethyl acetate (5) by UV, EI-MS, 1H and 13C-NMR. The antioxidant activity with the descending order was : verbascoside (1) (96.3 %) > rutin (2) (94.5 %) > phillygenin (4) (71.9 %) > ligustroside (3) (8.6 %) > p-hydroxyphenylethyl acetate (6) (5.5 %). Verbascoside and rutin were the major compounds with the strongest antioxidant activity, IC50 14.0 g/mL and 18.9 g/mL, respectively, and the highest content, 85.1 mg and 59.6 mg, in the O. fragrans flower extract. Chapter 3 aims to investigate the content of verbascoside and rutin in O. fragrans flower extract by HPLC. The calibration curve over a concentration range of 10~80 g/mL showed a good linearity, and with a correlation coefficient of 0.9974-0.9977. The results of inter-day and intra-day precision tests for the method have coefficient variation values of no more than 6.25 %. The average recovery rates of verbascoside and rutin in O. fragrans flower are 82.7% and 73.6 %, respectively, in accurate test. The concentration detection and quantization limits of the analysis method were determined to be 0.1 ng/mL. All results indicate that the HPLC analysis method proposed in this study is a sensitive, precise, and accurate method to determine concentrations of verbascoside and rutin in the O. fragrans flower and product. Then O. fragrans flower was extracted with different polarities solvent, and 75 % ethanol have the best antioxidant activity, total polyphenol content, and rutin content. The result revealed the relationship (correlation coefficient: 0.62~0.90) between the antioxidant activity and contents of total polyphenol , verbascoside and rutin. Verbascoside and rutin were the marker antioxidant on O. fragrans flower. Chapter 4 was evaluates the safety and potential functional application of O. fragrans flower extract and rutin. The oral treatment with 75 % ethanol extract of O. fragrans flower (100 and 1000 mg/kg mice) and rutin (10 and 100 mg/kg mice) for 28 consecutive day. No mortality was found in the 28 day feeding toxicity study. The weight grain rate and relative organ weight of lung, liver, kidney and spleen were no significant with normal control. The serum biochemical parameters, 1000 mg 75 % ethanol extract of O. fragrans flower was significantly increased GOT and GPT compared with normal control in serum. Liver histopathology showed that O. fragrans flower extract incidence of liver reversible infiltration. No toxicity, inflammatory and allergic reaction were found in the pulmonary histopathology and bronchoalveolar lavage fluid. The toxicity study of administration of O. fragrans flower extract orally daily the mice for 28 days indicated that the safe dose of O. fragrans flower extract was below 1000 mg/kg.