Generation and cloning of anti-polyethylene glycol antibody

• Main Authors: Sheng-Wen Hsiao, 蕭勝文
• Other Authors: Shiu-Ru Lin
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/72993943881333776458

碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 93 === The covalent bond the polyethylene glycol (polyethylene glycol, PEG) to protein improves its safety and the efficacy. PEG is one kind of water-solubility polymer. The matters after PEG modification for example: protein, hormone, and enzyme and cause to change the water solubility, reduces immunogenicity, increase the half-life, and change biodistribution. PEG modification drugs at present already had PEG-Intron (PEG-interferon alpha 2b), pegasys (PEG-IFN alpha 2a), PEG-Neupogen (PEG-G-CSF) and PEGV isomant (PEG-hGHra)that have been approved by the food and drug administration(FDA) and enter the clinical treatment or the experimental stage. Therefore qualitative and quantitative analysis PEG derivative member has its importance regarding the medicine development and the clinical practice. We have formerly made anti- PEG monoclonal antibody IgM —AGP3. Now we establish one kind of new anti-PEG monoclonal antibody IgG-E11. This project confirm E11 and AGP3 being able to bind on the PEG glycol skeleton and its length using western blot, ELISA and flow cytometry. Detection sensitivity increased with PEG length by sandwich ELISA includes the protein or the free state PEG.Accelerated clearance PEG modified protein in vivo, then will present used gene cloning technology to clone E11 and discuss as reporter gene or improve affinity.