| Summary: | 碩士 === 國立中興大學 === 昆蟲學系 === 93 === On the recently decade, the study is to investigate tripartite interactions between phytopathogens, plants, and herbivores which was rarely used crucifer and lepdioptera insect as the test materials. For this reason, cabbage (Brassica oleracea var. capitata L.), white cabbage butterfly (Pieris rapae crucivora (Boisduval)), and black leaf spot of crucifers (Alternaria brassicicola (Schw.)) were used as the test materials in this study. Larval long-term and short-term feeding trials, larval feeding and adult oviposition choice tests were conducted to assess the effect of B. oleracea was to inoculate with A. brassicicola on performance and behavior of P. rapae crucivora. The effect of plant leaf ages on performance of P. rapae crucivora was also investigated. To evaluate the effects of insect feeding on phytopathogen, the infected area of A. brassicicola on B. oleracea following of P. rapae crucivora was recorded. The results showed that P. rapae crucivora reared on B. oleracea was to inoculate with A. brassicicola which larval performance was poorer than which reared on B. oleracea was to inoculate without A. brassicicola. Results of two-way ANOVA indicated that B. oleracea was to inoculate with A. brassicicola and leaf age had significant effect on the performance of P. rapae crucivora. Furthermore, the results also indicated that B. oleracea was to inoculate with A. brassicicola and leaf age had interacting effect on duration (DUR), total consumption (TC), related consumption rate (RCR), approximate digestibility (AD), and efficiency of conversion of digest (ECD). However, there was no difference on leaf ages between B. oleracea was to inoculate with A. brassicicola and without A. brassicicola in feeding choice trials. Similar phenomenon was also occurred on the leaf 5th and 8th in oviposition preference test; but female of P. rapae crucivora preferred to oviposite on the 6th leaf of B. oleracea was to inoculate without A. brassicicola. Finally, infection area that was to inoculate with A. brassicicola on day 0 after larval damage was significant larger than those mechanical and control treatments. Nevertheless, no difference in infection area by A. brassicicola was found on other days’ results. Chemical assays revealed that no difference in nitrogen content, protein content, and peroxidase activity between B. oleracea was to inoculate with and without A. brassicicola. The results don’t completely explain the difference on performance and behavior of P. rapae crucivora, and need to more test to clear the effect of B. oleracea was to inoculate with A. brassicicola that affects P. rapae crucivora.
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