| Summary: | 碩士 === 國立中興大學 === 森林學系 === 93 === Summary
Taiwan red cypress (Chamaecyparis formosensis Matsum.) is an endemic precious tree specie grown in Taiwan. In this thesis, the bioactivities of extractives from Taiwan red cypress were investigated. Essential oil of Taiwan red cypress was prepared by water distillation and the chemical composition of essential oil was further analyzed by GC-MS. According to the results obtained from antimosquito and silverfish lethality assays, essential oil presented the good bioactivities against two kinds of organisms. It completely killed the larvae of A. aegypti and A. albopictus at the dosage of 100 μg/mL. As regard to silverfish lethality assay, we observed that the essential oil also had great empoisoned execution. While the concentration of essential oil dosage at 0.16 mg/cm3, the mortality was 100% after 2 hours treatment. In a parallel experiment, the wood chips were extracted with methanol. Its bioactivities were evaluated by using a number of bioassys, including brine shrimp test (BST), antioxidative activity assay and antifungal assay. In the meantime, the structures of the compounds, which isolated from total crude extract, were separated analyzed with chromatography as well as spectroscopy. The result of BST shown the extractives of Taiwan red cypress had the potential to further study their antitumor activity. The extractives also revealed significantly antifungal activity. The order of antifungal activities were EtOAc fraction >MeOH extract > EtOAc insolvable fraction. Following by chromatography separation and spectral analysis, four terpenoids were identified from active fraction, including T-Muurolol, 5α,7αH-eudesm-11(13)en-4α-ol, Isohinokiol and 6,12-De hydroxyabieta-5,8,11,13-tetraene-7-one. As regard to the antioxidative activity, the extractives of Taiwan red cypress heartwood did not possessed the good antioxidative activity so far. According to the results obtained from this study, the extractives of Taiwan red cypress possess multiple bioactivities. It is worthy to continue investigating its activity mechanisms and the exact structures of active components in the future.
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