A Pulsatile Perfusion Bioreactor for Vascular Tissue Engineering

• Main Authors: Yeu-Shiuan Lee, 李雨軒
• Other Authors: Da-Pan Chen
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/94280305516757020613

碩士 === 國立交通大學 === 機械工程系所 === 93 === In this study, we designed a bioreactor to provide the physical stimulation on the vascular tissue for the culturing. We used fibrin gel as tissue engineering scaffold. Due to the weak mechanical property of the gel. We add alginate to the fibrin gel to form fibrin-alginate gel. In this experiment, we concluded that both the perfusion and the pulsatile flow can cause the cell to attach firmly to the matrix, and secrete more extra-cellular matrix substance as in the human body environment. The fibrin-alginate gel can be applied to small caliber blood vessel tissue engineering. Together with the pulsatile perfusion system, the vascular tissue engineering proposed in this research is a success.