Biological Function Analysis of a Rice Cysteine Proteinase Gene, OsEP3A, by Gene Overexpression and Gene Silencing
碩士 === 國立嘉義大學 === 農業生物技術研究所碩士班 === 93 === Abstract OsEP3A (Oryza sativa endoproteinase 3 A) belongs to a gene family in rice genome and is known as a single copy. The expression of this gene is induced upon seed germination and leaf senescence. Furthermore, hormone and nitrogen source also regula...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2005
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| Online Access: | http://ndltd.ncl.edu.tw/handle/75888097568623841270 |
| Summary: | 碩士 === 國立嘉義大學 === 農業生物技術研究所碩士班 === 93 === Abstract
OsEP3A (Oryza sativa endoproteinase 3 A) belongs to a gene family in rice genome and is known as a single copy. The expression of this gene is induced upon seed germination and leaf senescence. Furthermore, hormone and nitrogen source also regulate its expression. The aim of this study was to test its function in transgenic rice plants. Specifically, gene constructs were made for overexpression or silencing for rice transformation via Agrobacteria mediated method.
A total of nine putative transgenic plants were obtained for the overexpression construct and eighteen putative transgenic plants for the silencing construct. It was found that the seeds of overexpression lines germinate earlier and their seedlings grow faster, as compared with the seeds from the silencing lines. However, the germination rates were comparable between the two transgenic lines. The most significant difference between the two lines was that the seedlings derived from the seeds of the silencing lines reveled earlier senescence and die after reaching 1-2 cm.
Genomic southern and northern blot analyses will be performed to confirm the copy number and transcript level in all T1 transgenic plants. Homozygous transgenic plants with one transgene copy will be used for its biological function analysis.
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