Use of C4 solid-phase extraction on-line with HPLC for the determination of 14 sulfonamides in ham

• Main Authors: LI, SHIN-YI, 李欣怡
• Other Authors: Chiu, Hung-Lin
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/71520447936600683078

碩士 === 國立高雄師範大學 === 化學系 === 93 === Abstract On-line solid phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) method was developed to determine sulfonamide in ham sample. The fourteen sulfonamides to be analyzed were sulfanilamide, sulfacetamide, sulfadiazine, sulfathiazole, sulfamerazine, sulfamethazine, sulfamethizole, sulfameter, sulfamonomethoxine, sulfachloropyridazine, sulfamethoxazole, sulfisoxazole, sulfadimethoxine, sulfaquinoxaline sodium salt. The study evaluated the optimal conditions of SPE conditions (sorbent , sample pH value, and the acid adding of the sample solution) and HPLC analyis conditions.The optimal conditions determined were applied to the real ham sample analysis. It is found that by using the developed method. The detection limit (LOD) were 0.2-0.7ppb, and precision is good. When it was used to detect fourteen sulfonamide in ham, we find all sulfonamides have good recovery (81-100%) except sulfanilamide (49%), so we can use simple calibration curve to quantify.