Effect of glutathione on porcine embryo development in vitro

• Main Authors: Wen Liang Liu, 劉文喨
• Other Authors: Yan Der Hsuuw
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/75087645507495777307

碩士 === 國立屏東科技大學 === 畜產系 === 94 === Objective of this study was to address the effects of glutathione (GSH) on the development of pig embryo following in vitro maturation and fertilization. Zygotes were either cultured in NCSU-23 or PZM-3 medium with or without 0.25 mM GSH for 5-7days. The development of embryos was examined by morphology, and total cell number on blastocysts was evaluated by orcein or differential staining. Results showed that total cell number of blastocysts is significantly higher in PZM-3 alone compared with GSH-supplemented NCSU-23 (P ＜ 0.05). To test hypothesis that GSH associated with PZM-3 medium might have a benefit effect on embryo growth, zygotes were cultured in PZM-3 medium containing 0, 0.25, 0.375 mM GSH for 5-7 days. Results showed that GSH has a dose-dependant increase on the blastocyst formation and total cell number of those emryos. GSH at 0.5 mM, however, has an inhibitory effect on blastocysts growth (P ＜ 0.05). Results from differential staining showed that effect of GSH on embryo growth is not selective on the inner cell mass of blastocysts. To learn the effect of GSH on improving the development of embryos derived from different quality of oocytes, zygotes from granulosa cumulus-oocyte complex (GCOCs) or cumulus-oocyte complex (COCs) were respectively cultured in the presence or absence of GSH (0.375 mM) for 5-7 days. Results showed that GSH has a significantly increase of the blastocyst formation on GCOCs but not on COCs (P ＜ 0.05). In conclusion, GSH at 0.375 mM supplemented in PZM-3 medium can improve the development of porcine zygotes. This enhance effect of GSH on embryo growth is more pronouncedly on GCOCs derived oocytes rather than COCs derived oocytes.