Pathological and Molecular Diagnosis on Primary Lymphoid Organ of Broiler Chickens with Emphases on Viral Immunosuppressive Diseases
碩士 === 國立屏東科技大學 === 獸醫學系 === 93 === Viral immunosuppressive diseases are commonly encountered in broiler chickens and usually lead to substantial economic losses. In addition, enhancement of secondary infections and failure of vaccinations are also frequently found in the immunocompromised chickens....
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ndltd-TW-093NPUST5410142016-12-22T04:11:08Z http://ndltd.ncl.edu.tw/handle/74929783247874635389 Pathological and Molecular Diagnosis on Primary Lymphoid Organ of Broiler Chickens with Emphases on Viral Immunosuppressive Diseases 肉雞免疫抑制病毒性疾病於初級淋巴器官之病理學暨分子生物學診斷 Shih-Yi Huang 黃詩伊 碩士 國立屏東科技大學 獸醫學系 93 Viral immunosuppressive diseases are commonly encountered in broiler chickens and usually lead to substantial economic losses. In addition, enhancement of secondary infections and failure of vaccinations are also frequently found in the immunocompromised chickens. The viral immunosuppressive diseases of broiler chickens manily include : Marek’s disease, chicken infectious anemia, infectious bursal disease, Newcastle Disease, pale bird syndrome and avian influenza. This study is to perform pathological and molecular diagnosis on primary lymphoid organs of the infected broiler chicken. Grossly, all the chickens have abnormal bursa and thymus. Depletion or necrosis of lymphocytes is the remarkable lesion, except that infiltration of lymphoid tumor cells is found in the cases of MDV infection. Two sets of multiplex polymerase chain reaction are developed for the simultaneous detection and differentiation of the immunosuppressive viruses. The first set is used to detect DNA viruses ( MDV and CIAV ) and the second is designed for RNA viruses ( NDV, AIV, REOV and IBDV ). In this study, DIG-labled riboprobes for the above pathogens are used to detect the sites of the agents in tissue sections. Postive signals are found in the cloacal bursa and thymus of all the cases examined, but only IBD, MD and CIA have signals in the bone marrow. Shinn-Shyong Tsai 蔡信雄 2005 學位論文 ; thesis 101 zh-TW |
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zh-TW |
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碩士 === 國立屏東科技大學 === 獸醫學系 === 93 === Viral immunosuppressive diseases are commonly encountered in broiler chickens and usually lead to substantial economic losses. In addition, enhancement of secondary infections and failure of vaccinations are also frequently found in the immunocompromised chickens. The viral immunosuppressive diseases of broiler chickens manily include : Marek’s disease, chicken infectious anemia, infectious bursal disease, Newcastle Disease, pale bird syndrome and avian influenza. This study is to perform pathological and molecular diagnosis on primary lymphoid organs of the infected broiler chicken. Grossly, all the chickens have abnormal bursa and thymus. Depletion or necrosis of lymphocytes is the remarkable lesion, except that infiltration of lymphoid tumor cells is found in the cases of MDV infection. Two sets of multiplex polymerase chain reaction are developed for the simultaneous detection and differentiation of the immunosuppressive viruses. The first set is used to detect DNA viruses ( MDV and CIAV ) and the second is designed for RNA viruses ( NDV, AIV, REOV and IBDV ). In this study, DIG-labled riboprobes for the above pathogens are used to detect the sites of the agents in tissue sections. Postive signals are found in the cloacal bursa and thymus of all the cases examined, but only IBD, MD and CIA have signals in the bone marrow.
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author2 |
Shinn-Shyong Tsai |
author_facet |
Shinn-Shyong Tsai Shih-Yi Huang 黃詩伊 |
author |
Shih-Yi Huang 黃詩伊 |
spellingShingle |
Shih-Yi Huang 黃詩伊 Pathological and Molecular Diagnosis on Primary Lymphoid Organ of Broiler Chickens with Emphases on Viral Immunosuppressive Diseases |
author_sort |
Shih-Yi Huang |
title |
Pathological and Molecular Diagnosis on Primary Lymphoid Organ of Broiler Chickens with Emphases on Viral Immunosuppressive Diseases |
title_short |
Pathological and Molecular Diagnosis on Primary Lymphoid Organ of Broiler Chickens with Emphases on Viral Immunosuppressive Diseases |
title_full |
Pathological and Molecular Diagnosis on Primary Lymphoid Organ of Broiler Chickens with Emphases on Viral Immunosuppressive Diseases |
title_fullStr |
Pathological and Molecular Diagnosis on Primary Lymphoid Organ of Broiler Chickens with Emphases on Viral Immunosuppressive Diseases |
title_full_unstemmed |
Pathological and Molecular Diagnosis on Primary Lymphoid Organ of Broiler Chickens with Emphases on Viral Immunosuppressive Diseases |
title_sort |
pathological and molecular diagnosis on primary lymphoid organ of broiler chickens with emphases on viral immunosuppressive diseases |
publishDate |
2005 |
url |
http://ndltd.ncl.edu.tw/handle/74929783247874635389 |
work_keys_str_mv |
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