Effect of Indole-3-butyric Acid on the Formation of Adventitious Roots in Cinnamomum kanehirae Cuttings

• Main Authors: Chiung-yun Chang, 張瓊云
• Other Authors: Zin-huang Liu
• Format: Others
• Language: en_US
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/88869556006243271973

碩士 === 國立中山大學 === 生物科學系研究所 === 93 === Synthetic auxin, indole-3-butyric acid (IBA), effectively promoted the rooting in Cinnamomum kanehirae cuttings. The easy-to-root genotype, H107, responded to IBA much earlier than the difficult-to-root genotype, L41. On day 5, the POD activity significantly decreased in the IBA-treated tissues as compared with the control. Similar phenomenon was observed in extract of L41 genotype showing that IBA inhibited POD activity. In addition, the variation in POD activity corresponds to an inverse variation in the concentration of free IAA. The levels of IAA in H107 genotype increased dramatically in IBA-treated tissues on day 5; while, in L41 genotype, the raise of IAA in IBA-treated tissues was observed on day 20. Moreover, the lignin content in IBA-treated H107 cuttings decreased is quite correlated with the decline of the POD activity; yet the lignin content in L41 genotype cuttings was almost unchanged. Hence, we suggest that the inhibition on POD may lead to the redifferentiation processes induced by IBA, then produce the new root primordia during the formation of adventitious roots.