Molecular characterization on a t(1;1)(p13;p36) acute megakaryoblastic leukemia (AMKL)

• Main Authors: Ya-lan Hsieh, 謝亞嵐
• Other Authors: Yow-Ling Shiue
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/46812087322018730411

碩士 === 國立中山大學 === 生物醫學科學研究所 === 93 === Acute megakaryoblastic leukemia (AMKL) was first described by Von Boros and Karangi in 1931, was a result of developments in ultrastructural cytochemistry and immunologic phenotyping acute myeloid leukemia (AML) of megakaryocytic lineage have been diagnosed increasingly. The French-American-British (FAB) Co-operative Group established the criteria for the diagnosis and added this category as a distinct subtype of AML (M7) in 1985. The main subtypes of AML in the infants are M4, M5, and M7. One 25-day-old infant was referred to the hospital for further examination of white blood cell. Hepatosplenomegaly and anemia were physically examined, and he was diagnosed to be an AMKL case. Abnormal karyotype 46,XY,t(1;1)(p13;p36) was observed in this patient. This study aims to identify the AMKL potentially related genes on the breakpoints of Homo sapiens autosomal (HSA) 1p13 and 1p36 in this case by candidate gene approaches. Data-mining of the AMKL potentially related genes on breakpoints of HSA1p13 and 1p36 through NCBI Map Viewer Database, OMIM Morbid Map, and OMIM Gene Map were performed. We identified three candidate genes on HSA1p13 and 15 candidate genes on HSA 1p36. RBM15-MKL1 fusion on t(1;22)(p13;q13) was reported to be AMKL genes by Ma et al., Mercher et al., and the Mitelman Database of Chromosome Aberrations in Cancer. We anticipated RBM15 is also a related gene on HSA1p13 in this AMKL case, and compared the Gene Ontology terms between MKL1 and these 15 candidate genes on HSA1p36. SKI becomes our first candidate gene on 1p36 in this case. To identify candidate genes locating at HSA1p13 and 1p36, including RBM15 and SKI were screened at both cDNA and genomic DNA levels. According to these results, RBM15 and SKI are more likely to be candidate genes. Thus RBM15 and SKI may be the novel AMKL genes in t(1;1)(p13;p36) AMKL patients.