Micro Rayleigh-Bénard polymerase chain reaction system

• Main Authors: Jhao-Rong Chen, 陳昭榮
• Other Authors: Da-Jeng Yao
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/05888212591754965876

碩士 === 國立清華大學 === 工程與系統科學系 === 93 === Polymerase chain reaction (PCR) is a molecular biological method for the in vitro amplification of nucleic acid molecule. In this thesis, the research object was to design a micro PCR system which involved a Rayleigh-Bénard convection PCR chip, measurement circuits, and temperature control circuits. Rayleigh-Bénard convection PCR chip was easy to be fabricated, and the sample solution in it can transit its temperature immediately. Thus, the faster the speed of flow is, the higher heating and cooling rate is. Rayleigh-Bénard convection PCR chip can be divided into two parts. First part is a chip with micro heaters and micro temperature sensors. Second part is PDMS reaction chamber designed by Rayleigh-Bénard convection theory. Temperature control system is designed to keep temperature on the top and bottom chips by Pulse width modulation control. Analog temperature signal is precisely measured by circuits. Then the signal is processed by an 8051 single chip, and the output power of micro heaters is controlled to adjust the temperature of top and down plates. Finally, agarose gel electrophoresis is used to verify the practicability of Rayleigh-Bénard convection PCR system. By comparing with the PCR experiments done by the commercial PCR machine and our chips, our chips can plenty decrease the reaction time. And By comparing with the simulation and PCR experiments of different designed sizes, users can use setted parameters and CFD results to do optimal designs and then decrease the total reaction time in the future.