| Summary: | 碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 93 === The purpose of this thesis was evaluate the effect of vitamin C and β-glucan on the non-specific immune response of grouper(Epinephelus coioides) by oral administration. Therefore, the non-specific immune immunological parameters and physiology parameters such as the superoxide anion (O2-) production ratio, superoxide dismutase activity (SOD), phagocytic rate, phagocytic indexes, albumin, globulin, albumin/globulin ratio (A/G ratio), glutamate oxaloacetate transaminase activity (GOT) and glutamate pyruvate transaminase activity (GPT).
The result showed that superoxide anion, peroxide dismutase activity and phagocytosis were significantly enhanced in groupers fed the with diets containing vitamin C and/or β-glucan during sixteen days. Therefore, it is demonstrated that vitamin C and/or β-glucan are useful immunostimulants. The albumin/globulin ratio gradually decreased in serum after groupers fed the experimental diets containing vitamin C , mixture of β-glucan and vitamin C or β-glucan along respectively. When albumin was increased, globulin was also increased. On the other hand, albumin was decreased, globulin was also decreased. This result indicated that vitamin C and/or β-glucan can’t increase immunoglobulin of grouper. The enzyme activity of GOT and GPT gradually decreased in serum after groupers fed the experimental diets containing vitamin C or mixture of β-glucan and vitamin C. This result indicated that vitamin C were helpful to wound healing of grouper.
The result also showed that the groupers had higher non-specific immune response when vitamin C was used together with β-glucan. And the groupers fed with mixture of 2g/kg vitaimin C and 2g/kg β-glucan were significantly enhanced non-specific immune immunological parameters from 4-16 days.
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