Regulation of brain 5-HydroxyTryptamine in black porgy, Acanthupagrus schlegeli.

• Main Authors: Sue-Shin Fong, 馮素��
• Other Authors: Chang ching-fong
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/06841036735278064058

碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 93 === The objectives of the study were to analyze the brain neurotransmitters in black porgy, Acanthopagrus schlegeli, and also to study the effects of brain neurotransmitters on the endocrine system, especially on the hypothalamus-pituitary-gonad axis during the reproctive season and non-reproductive season. During non-reproductive season, treatment with estradiol (E2 1.5 µg/g BW) slightly increased the brain 5-HT contert；but during reproductive season, they were no regulatory effect on brain 5-HT content by E2. During non-reproductive season, treatment with LHRH analog, E2 increased brain 5-HT content. But during reproductive season, LHRH analog did not regulate brain 5-HT content. Besides the research on 5-HT content, we also cloned black porgy tyrosine hydroxylase gene. The homolog were 97% with gilthead seabream and 80% with European eel.