| Summary: | 碩士 === 國立臺灣大學 === 微生物與生化學研究所 === 93 === The phenomenon of bamboo flowering and the following death is a mystery to plant biologist. In order to explicit this problem in molecular level, we tried to understand the mechanism of bamboo flowering. We used two-dimensional electrophoresis in conjunction with mass spectrometry to dissect the proteome of flower buds and basal shoot clumps of Bambusa oldhamii which were cultivated in vitro. Proteomic tools including two-dimensional electrophoresis, immunoblotting, computer software comparing, CyDye tagging and mass spectrometric analysis were used to identify the key proteins related to the flowering mechanism. It is observed the expression of the enzymes responsible for amino acids, carbohydrates and fatty acids metabolism were changed. The increase of these proteins could result in flux toward glycolysis, TCA cycle, photosynthesis and fatty acid synthesis. Besides, we have identified some enzymes related to antioxidation osmotic stress which might regulate plant growth by co-regulating with the phytohormones in flower buds. All these observations should be confirmed in further studies.
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