Studies on the production of prodigiosin by Serratia marcescens BCRC 11576

• Main Authors: Yin-An Chen, 陳盈安
• Other Authors: Ming-Hsiung Lee
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/33029867992974433372

碩士 === 國立臺灣大學 === 農業化學研究所 === 93 === Abstract The structure of prodigiosin is a linar tripyrrole which is a typical secondary metabolite produced by Serratia marcesens, Vibrio psychroerythrus, Pseudomonas magnesiorubra, and Streptomyces spp. The characteristics of prodigiosin families were reported characteristics of having not only having antibiotics and immunosuppressant activities, but also given anticancer activity recently. In this study, the strain, Serratia marcescens BCRC 11576, was used which cultured on a natural medium to produce prodigiosin. In addition, in order to effective to prevent pH in the period of cultivation decrease the culture was added with 0.5 % CaCO3. Finally, a complete natural medium was composed of 1.4 % soybean flour, 0.5 % CaCO3, 0.6% fructose, 0.1 % KCl, 0.05 % MgSO4•7H2O,and 0.2 % wheat flour was established. This medium induced formation of 81.99 mg prodigiosin per 100 mL broth after incubation for two days, and 96.86 mg prodigiosin per 100mL broth for three days under 27 ℃, 120 rpm. Therefore, it seemed so economic a way to produce prodigiosin with this medium.